) [taxonomy] => category [taxonomy] => category [117] => WP_Term Object [name] => AppNotes Cogent Silica-C [filter] => raw [term_id] => 124 ) [term_id] => 145 [filter] => raw ( [taxonomy] => category [customExpand] => [description] => ( [filter] => raw For example, 1 L of ammonium acetate (buffer 20, mM pH 4.0) will be prepared the following way: 100 mmol of ammonium acetate salt is dissolved in a little less than 1000 mL of water, the acidity of the solution is adjusted to pH=4.0 by (adding) acetic acid and total volume is adjusted to 1000 mL with water. [parent] => 153 [taxonomy] => category [count] => 8 Adjust pH to the target value by using either diluted acetic acid or a diluted ammonium hydroxide solution. [taxonomy] => category [slug] => 148 [slug] => 167 [term_id] => 242 [filter] => raw [count] => 26 [term_id] => 249 [taxonomy] => category [slug] => 28 [name] => Bidentate C18 FAQ ( [description] => [filter] => raw [count] => 5 ( ) [term_taxonomy_id] => 164 [count] => 4 [name] => Prep Chromatography ( [description] => [slug] => 84 [name] => Click To Chat Internal [name] => Crimp Top Vials & Cap FAQ [filter] => raw [term_group] => 0 [term_group] => 0 [name] => Customer Care Department These ambiguities can lead to inconsistencies in the way the mobile phase is prepared and hence in the resulting data. ( Preparation of 10mM Ammonium acetate buffer (1Liter): 1. [term_taxonomy_id] => 103 [description] => ) [filter] => raw [filter] => raw Rinse into your flask with enough water to ensure a complete transfer of the buffering salts. ( [term_id] => 238 [term_id] => 150 [filter] => raw [slug] => 75 When you see something like “10 mM ammonium acetate in 90:10 acetonitrile : buffer” in a method, you may be wondering how exactly this mobile phase solution is prepared. ( [description] => ) [slug] => 82 [taxonomy] => category [parent] => 15 [term_id] => 14 [taxonomy] => category [name] => SAGE 100 [filter] => raw [59] => WP_Term Object [name] => CElixir Products [parent] => 153 ) [slug] => 115 [filter] => raw [description] => [term_group] => 0 [term_group] => 0 [slug] => 45 [count] => 2 [count] => 34 [count] => 6 [description] => [filter] => raw [term_group] => 0 [count] => 4 [filter] => raw [filter] => raw [slug] => 61 [taxonomy] => category [term_group] => 0 [parent] => 153 [count] => 3 Dilute to mark with DI water. [parent] => 4 [term_group] => 0 [term_taxonomy_id] => 216 [slug] => 111 [name] => Diamond Hydride FAQ Buffer: 5 mM ammonium acetate pH 5.8, 5 mM effective concentration* Channel A: 90/10 acetonitrile/50 mM ammonium acetate pH 5.8 Channel B: 50/40/10 acetonitrile /water/50 mM ammonium acetate pH 5.8 0 10 20 30 40 50 60 70 % change Acetylcholine % change Serotonin % change Dopamine 12. [term_taxonomy_id] => 150 [filter] => raw [postTitleLength] => 0 [term_group] => 0 Recipe can be automatically scaled by entering desired final volume. ( [term_id] => 258 [term_taxonomy_id] => 236 [term_taxonomy_id] => 148 [parent] => 7 [45] => WP_Term Object [term_group] => 0 ( [description] => [description] => [description] => [count] => 16 [filter] => raw [name] => White Papers [count] => 357 [title_link] => ) [filter] => raw [taxonomy] => category [description] => ) [term_taxonomy_id] => 165 [term_id] => 257 ) [term_group] => 0 [70] => WP_Term Object [taxonomy] => category [term_taxonomy_id] => 127 [name] => Pre Column HPLC Filters FAQ [name] => Fittings for HPLC FAQ [name] => AppNotes Listed by Discipline [term_id] => 156 ) [taxonomy] => category ( [term_id] => 162 Any compounds, such as ammonium acetate, tend to resist any change in their concentration of hydronium ions or pH, whenever a small amount of a strong acid or a strong base is applied to it. This makes your mobile phase solution “10 mM ammonium acetate in 90:10 acetonitrile : buffer“ These instructions ensure you get reproducible results with respect to the mobile phase. [term_taxonomy_id] => 128 [slug] => 86 ) ) Label the reservoir bottle "20 mM Ammonium Formate Buffer pH 10". ( ( [81] => WP_Term Object ) [filter] => raw [filter] => raw [term_id] => 106 [slug] => 117 [term_taxonomy_id] => 157 [name] => Partisil & Partisphere FAQ [name] => Sales Order Module [term_id] => 233 [description] => [description] => [filter] => raw [slug] => 107 Buffer. [slug] => 100 [filter] => raw [parent] => 210 [term_group] => 0 [parent] => 205 [taxonomy] => category [count] => 3 ) [description] => [term_id] => 127 [parent] => 84 Acetate Buffer pH 6.0: Dissolve 100 g of ammonium acetate in 300 ml of water, add 4.1 ml of glacial acetic acid, adjust the pH, if necessary, using 10M ammonia or 5 M acetic acid and dilute with water to 500 ml. 2. [term_id] => 256 [count] => 189 [count] => 11 ) [133] => WP_Term Object [term_group] => 0 ( [taxonomy] => category I want to prepare 0.02 M ammonium acetate buffer (pH:9); this needs 7.719 g of sodium acetate (anhydrous) to 800 mL distilled water, and 0.353 g of acetic acid. This large drop reflects the fact that the initial pH is not within the buffering range of pK a ± 1 pH unit. [count] => 11 [term_id] => 152 ( [filter] => raw [filter] => raw [count] => 2 [term_group] => 0 ) [filter] => raw [filter] => raw [showEmptyCat] => 1 From my link one gets: [term_taxonomy_id] => 78 [term_taxonomy_id] => 100 ( [count] => 9 ) [29] => WP_Term Object [15] => WP_Term Object [term_id] => 101 ( [count] => 5 [slug] => 74 [74] => WP_Term Object [parent] => 119 [count] => 43 [term_id] => 163 [description] => [description] => [term_id] => 103 [58] => WP_Term Object [term_taxonomy_id] => 219 [85] => WP_Term Object ) [description] => [term_group] => 0 ( [description] => [term_taxonomy_id] => 98 [description] => ) [count] => 2 ) Phone: +1 732 380 8900 ) [name] => Technical Support Dept Internal [name] => General Policies & Procedure Internal [count] => 23 [parent] => 7 [count] => 3 [taxonomy] => category [collapseSym] => [name] => Accounting - AP/AR Department [count] => 2 How do you prepare 10mM ammonium acetate buffer? ( [slug] => 87 [slug] => 9 This makes a 100 mM solution. Preparation: It is prepared by mixing a weak base and its salt of strong acid in a water medium. [slug] => 138 [slug] => 13 ( [term_id] => 84 [count] => 8 [slug] => 94 ( [filter] => raw The pH of the solution was adjusted to pH 7.0 by adding ) [term_group] => 0 [term_taxonomy_id] => 142 [slug] => 110 [taxonomy] => category [term_id] => 73 [126] => WP_Term Object [term_id] => 232 ( [term_group] => 0 [118] => WP_Term Object [term_group] => 0 [count] => 13 [slug] => 73 [parent] => 14 [term_id] => 119 [91] => WP_Term Object [taxonomy] => category ) [slug] => 40 [term_taxonomy_id] => 167 [20] => WP_Term Object [description] => [filter] => raw [term_id] => 206 [parent] => 84 [21] => WP_Term Object [parent] => 147 Buffer solutions are typically prepared by dissolving an appropriate salt in water and then adjusting the $\mathrm{pH}$ to the desired position by addition of the conjugate acid or base.. For example, to prepare a $\pu{100 mM}$ ammonium acetate solution at $\mathrm{pH}~9,$ you’d first prepare a $\pu{100 mM}$ ammonium acetate, and then add ammonium hydroxide … [taxonomy] => category [description] => A solution which has a stable pH is termed as a buffer solution. [filter] => raw ) [term_taxonomy_id] => 102 [term_id] => 110 [count] => 14 ( [parent] => 210 [slug] => celixir-products [term_taxonomy_id] => 235 [slug] => 76 [parent] => 5 [description] => [term_id] => 218 [filter] => raw )      3. 90/10 Mixture: Pipet 100 mL Buffer Solution into a 1000 mL volumetric flask and dilute to mark with acetonitrile. ( ( [parent] => 71 This makes a 10 mM solution. [parent] => 210 [term_group] => 0 [name] => AppNotes HPLC [parent] => 7 [name] => MicroSolv Autosampler Vials FAQ [filter] => raw [term_taxonomy_id] => 162 [parent] => 4 [debug] => 1 [term_taxonomy_id] => 70 [count] => 15 [term_taxonomy_id] => 71 ) [32] => WP_Term Object [name] => Cogent HPS FAQ [10] => WP_Term Object [term_taxonomy_id] => 237 [taxonomy] => category [107] => WP_Term Object ) [filter] => raw [parent] => 128 ) [slug] => 155 [69] => WP_Term Object [name] => AppNotes Cogent Diamond Hydride [term_taxonomy_id] => 118 [count] => 17 [term_group] => 0 [term_group] => 0 The addition of 1 mM H + decreases the pH to 5.8. [term_group] => 0 [description] => [description] => [description] => [82] => WP_Term Object [slug] => 15 4. [term_taxonomy_id] => 5 [123] => WP_Term Object [description] => The product is water containing 10mM ammonium acetate that can be employed for HPLC. Prepare a 200 mM stock buffer of ammonium acetate by dissolving 15.4 g of ammonium acetate and adding it to 900 mL of water in a 1 L volumetric flask. What is the relationship of egret and carabao? ) ) These instructions ensure you get reproducible results with respect to the mobile phase. [parent] => 153 [filter] => raw [slug] => 23 1 M Dithioth r eitol (DTT) Dissolve 1.5g of DTT (DL-dithiothreitol, anhydrous m.w.=154.25) in 8 ml of deionized or distilled H20 ) [slug] => 85 [term_group] => 0 [term_id] => 229 [term_taxonomy_id] => 126 [taxonomy] => category [excludeAll] => 0 [term_taxonomy_id] => 138 [term_taxonomy_id] => 223 [19] => WP_Term Object ( [count] => 29 [term_id] => 225 [term_taxonomy_id] => 18 [name] => AppNotes RSA Vials [count] => 4 ( Weigh your buffer salt in a weigh boat and transfer to a volumetric flask. [count] => 1 Fig 87. [filter] => raw [description] => [term_group] => 0 [postDateFormat] => m/d [count] => 1 [parent] => 4 Adjust volume to 1 L with H 2 O. Sterilize by filtration. [description] => ) [term_id] => 75 [description] => [slug] => cogent-type-c-silica-columns [slug] => 99 [slug] => 171 [slug] => 168 ( [filter] => raw Adjust solution to final desired pH using HCl or NaOH Add distilled water until volume is 1 L. [taxonomy] => category [parent] => 84 [filter] => raw My preferred last step is to rinse with purified water and drip dry. [slug] => 172 [parent] => 153 [term_id] => 236 [slug] => 52 [description] => 2. [count] => 2 [description] => [term_id] => 70 work, phosphate, cannot be used. ( [description] => ) ( [term_group] => 0 Do you just mix 900 mL acetonitrile and 100 mL buffer? [term_id] => 217 [filter] => raw [name] => RSA Vials FAQ [slug] => 49 [49] => WP_Term Object [term_group] => 0 [term_taxonomy_id] => 171 [slug] => 78 [name] => Syringe Filters FAQ ) [term_group] => 0 ( [term_id] => 114      2. Buffer Solution (10 mM Ammonium acetate aqueous solution): Pipet 100 mL Stock Solution into a 1000 mL volumetric flask and dilute to mark with DI water. [term_group] => 0 [term_taxonomy_id] => 135 [name] => Trouble Shooting Suggestions [term_taxonomy_id] => 106 ) [slug] => 104 [term_group] => 0 [name] => Silica-C FAQ [description] => [description] => [name] => Inserts FAQ [taxonomy] => category [taxonomy] => category [term_group] => 0 [count] => 1176 Buffer exchange of mouse plasma (10 ml) on HiPrep™ 26/10 Desalting. ( [94] => WP_Term Object [taxonomy] => category [term_taxonomy_id] => 96 [description] => ( [132] => WP_Term Object [term_id] => 76 [count] => 25 ( [filter] => raw [term_taxonomy_id] => 225 [name] => Amide FAQ [filter] => raw [taxonomy] => category [count] => 1 ) [count] => 1 ( ) [name] => 2.o™ HPLC Columns FAQ [109] => WP_Term Object [taxonomy] => category ( [taxonomy] => category [count] => 2 [count] => 17 [term_taxonomy_id] => 17 [taxonomy] => category Ammonium formate (2.7 Mobile Phase FAQ [term_group] => 0 [term_group] => 0 [name] => Chrom Syringes FAQ [parent] => 139 [name] => AppNotes Cogent Bidentate C18 ( [term_id] => 109 [term_taxonomy_id] => 249 [slug] => 146 [term_group] => 0 [term_group] => 0 [name] => Financial Reporting [25] => WP_Term Object [filter] => raw [filter] => raw [term_id] => 220 [description] => [name] => Sales Department Outbound [taxonomy] => category [count] => 15 [name] => HPLC Columns FAQ [filter] => raw ( ( with acetonitrile added) or just with the aqueous component? ) [name] => SALES POLICIES ) [filter] => raw ( [parent] => 5 [term_group] => 0 [taxonomy] => category ( [slug] => 141 [term_id] => 94 [filter] => raw
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